Part:BBa_K4927005

CL7

Introduction

Our team designed truncated mutants CL7, which were inactivated by E. coli CE7 nuclease. Based on the high similarity of amino acid sequences between CE2, CE8, and CE9 and CE7, corresponding CE2, CE8, and CE9 truncated mutants CL2, CL8, and CL9 were designed, and corresponding inhibitors Im2, Im7, Im8, and Im9 were obtained to construct an artificial protein scaffold Scaf-CIQ

Figure1:a.CE7/IM7 reaction pairs; B.CE7/DNA reaction pair;C.CE7/CL7 protein sequence alignment

Usage and Biology

The CL7 tag will bind to formaldehyde dehydrogenase (FALDH) to form complex T7 and F7(See the figure below）

Figure2:The strcture of T7

Figure3:The strcture of F7

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]